Journal: Cells

Article Title: Activin A, a Novel Chemokine, Induces Mouse NK Cell Migration via AKT and Calcium Signaling

doi: 10.3390/cells13090728

Figure Lengend Snippet: Effects of activin A on F-actin polarization and expression of migration-related proteins of mouse NK cells. ( A ) The nuclei were stained with DAPI (blue) and the cell was stained with FITC-labeled phalloidin that preferentially labels filamentous actin (F-actin) (green). NK cells (white arrows) were treated with culture medium (Control), 20 ng/mL activin A, 200 ng/mL CXCL12 or 20 ng/mL activin A + 200 ng/mL CXCL12. Scale bar = 10 µm. ( B ) Levels of β-catenin, vimentin and MMP2 protein in mouse NK cells were examined by Western blotting, after treating the cells with culture medium (lane 1), 10 ng/mL activin A (lane 2), 100 ng/mL CXCL12 (lane 3) or 10 ng/mL activin A + 100 ng/mL CXCL12 (lane 4) for 2 h. The levels of protein expression were normalized against those of GAPDH ( n = 3). * p < 0.05; ** p < 0.01, compared with the control group (one-way ANOVA; mean ± S.D.).

Article Snippet: Rabbit polyclonal antibodies against vimentin (Cat# bs-0756R) were obtained from Bioss (Beijing, China).

Techniques: Expressing, Migration, Staining, Labeling, Control, Western Blot

Journal: Antioxidants (Basel, Switzerland)

Article Title: MOMAST ® Downregulates AQP3 Expression and Function in Human Colon Cells.

doi: 10.3390/antiox14010026

Figure Lengend Snippet: Figure 9. Effect of MOMAST® on EMT markers expression in HCT8 cells. Treatments with MOMAST® at increasing concentrations induced a significant reduction in the EMT proteins expres- sion β-catenin (a) and vimentin (b) likely suggesting a possible role of MOMAST® in modulating the expression level of selective EMT protein markers. Data are shown as means ± Standard Error Means (S.E.M.) of four independent experiments and analyzed with one-way ANOVA followed by Dunnett’s multiple comparisons test (* p < 0.05 vs. CTR; ** p < 0.01 vs. CTR; *** p < 0.001 vs. CTR).

Article Snippet: The rabbit polyclonal antibody against vimentin was purchased from Proteintech (Cat# 10366-1-AP, Proteintech Group, Inc., Rosemont, IL, USA).

Techniques: Expressing

Journal: Antioxidants (Basel, Switzerland)

Article Title: MOMAST ® Downregulates AQP3 Expression and Function in Human Colon Cells.

doi: 10.3390/antiox14010026

Figure Lengend Snippet: Scheme 2. Model depicting the effects of MOMAST® exerted in HCT8 cells. Specifically, MOMAST®- reduced AQP3 expression and function and affect the expression level of the EMT protein markers, vimentin and β-catenin. The downward arrow indicates a reduction.

Article Snippet: The rabbit polyclonal antibody against vimentin was purchased from Proteintech (Cat# 10366-1-AP, Proteintech Group, Inc., Rosemont, IL, USA).

Techniques: Expressing

Journal: Parasites & Vectors

Article Title: Effects of host vimentin on Eimeria tenella sporozoite invasion

doi: 10.1186/s13071-021-05107-4

Figure Lengend Snippet: Sequences of siRNA used to downregulate the vimentin expression

Article Snippet: The PVDF membrane was blocked in 5% skim milk for 2 h at 37 °C and then incubated with polyclonal rabbit antibody against vimentin (1:200, prepared by our laboratory) or β-GAPDH (internal reference, 1:2000; Yeasen, Shanghai, China) for 2 h at 37 °C.

Techniques:

Journal: Parasites & Vectors

Article Title: Effects of host vimentin on Eimeria tenella sporozoite invasion

doi: 10.1186/s13071-021-05107-4

Figure Lengend Snippet: Vimentin mRNA transcription levels in E. tenella sporozoite-infected DF-1 cells. * P < 0.05, ** < 0.01 and *** < 0.001 by Student’s t- test compared with the uninfected group

Article Snippet: The PVDF membrane was blocked in 5% skim milk for 2 h at 37 °C and then incubated with polyclonal rabbit antibody against vimentin (1:200, prepared by our laboratory) or β-GAPDH (internal reference, 1:2000; Yeasen, Shanghai, China) for 2 h at 37 °C.

Techniques: Infection

Journal: Parasites & Vectors

Article Title: Effects of host vimentin on Eimeria tenella sporozoite invasion

doi: 10.1186/s13071-021-05107-4

Figure Lengend Snippet: Vimentin protein expression levels in E. tenella sporozoite-infected DF-1 cells. a Western blotting of the internal reference GAPDH and vimentin protein. b Gray analysis of the relative expression level of the vimentin protein. ** P < 0.01 by Student’s t -test compared with the uninfected group

Article Snippet: The PVDF membrane was blocked in 5% skim milk for 2 h at 37 °C and then incubated with polyclonal rabbit antibody against vimentin (1:200, prepared by our laboratory) or β-GAPDH (internal reference, 1:2000; Yeasen, Shanghai, China) for 2 h at 37 °C.

Techniques: Expressing, Infection, Western Blot

Journal: Parasites & Vectors

Article Title: Effects of host vimentin on Eimeria tenella sporozoite invasion

doi: 10.1186/s13071-021-05107-4

Figure Lengend Snippet: Total phosphorylation levels of vimentin in E. tenella sporozoite-infected DF-1 cells. a Western blotting of phosphorylated and unphosphorylated vimentin with a consistent level of total vimentin. b Gray analysis of the relative expression level of phosphorylated vimentin. * P < 0.05 and ** P < 0.01 Student’s t -test compared with the uninfected group

Article Snippet: The PVDF membrane was blocked in 5% skim milk for 2 h at 37 °C and then incubated with polyclonal rabbit antibody against vimentin (1:200, prepared by our laboratory) or β-GAPDH (internal reference, 1:2000; Yeasen, Shanghai, China) for 2 h at 37 °C.

Techniques: Phospho-proteomics, Infection, Western Blot, Expressing

Journal: Parasites & Vectors

Article Title: Effects of host vimentin on Eimeria tenella sporozoite invasion

doi: 10.1186/s13071-021-05107-4

Figure Lengend Snippet: Changes in vimentin distribution after E. tenella sporozoite invasion in DF-1 cells

Article Snippet: The PVDF membrane was blocked in 5% skim milk for 2 h at 37 °C and then incubated with polyclonal rabbit antibody against vimentin (1:200, prepared by our laboratory) or β-GAPDH (internal reference, 1:2000; Yeasen, Shanghai, China) for 2 h at 37 °C.

Techniques:

Journal: Parasites & Vectors

Article Title: Effects of host vimentin on Eimeria tenella sporozoite invasion

doi: 10.1186/s13071-021-05107-4

Figure Lengend Snippet: Effect of anti-vimentin polyclonal antibody on E. tenella sporozoite invasion in DF-1 cells. ** P < 0.01 by Student’s t -test compared with anti-vimentin polyclonal antibody treatment or with the same dose of normal rabbit IgG

Article Snippet: The PVDF membrane was blocked in 5% skim milk for 2 h at 37 °C and then incubated with polyclonal rabbit antibody against vimentin (1:200, prepared by our laboratory) or β-GAPDH (internal reference, 1:2000; Yeasen, Shanghai, China) for 2 h at 37 °C.

Techniques:

Journal: Parasites & Vectors

Article Title: Effects of host vimentin on Eimeria tenella sporozoite invasion

doi: 10.1186/s13071-021-05107-4

Figure Lengend Snippet: Effects of vimentin downregulation on sporozoite invasion in DF-1 cells. a The expression of vimentin protein in different transfection groups. b Cell growth curves of different transfection groups. c The invasion rate of sporozoites after downregulation of vimentin. ** P < 0.01 by Student’s t -test compared with downregulated vimentin expression level or the untreated group

Article Snippet: The PVDF membrane was blocked in 5% skim milk for 2 h at 37 °C and then incubated with polyclonal rabbit antibody against vimentin (1:200, prepared by our laboratory) or β-GAPDH (internal reference, 1:2000; Yeasen, Shanghai, China) for 2 h at 37 °C.

Techniques: Expressing, Transfection

Journal: Parasites & Vectors

Article Title: Effects of host vimentin on Eimeria tenella sporozoite invasion

doi: 10.1186/s13071-021-05107-4

Figure Lengend Snippet: Sequences of siRNA used to downregulate the vimentin expression

Article Snippet: Polyclonal rabbit antibody against vimentin (prepared by our laboratory) was purified using protein A+G agarose (Beyotime).

Techniques:

Journal: Parasites & Vectors

Article Title: Effects of host vimentin on Eimeria tenella sporozoite invasion

doi: 10.1186/s13071-021-05107-4

Figure Lengend Snippet: Vimentin mRNA transcription levels in E. tenella sporozoite-infected DF-1 cells. * P < 0.05, ** < 0.01 and *** < 0.001 by Student’s t- test compared with the uninfected group

Article Snippet: Polyclonal rabbit antibody against vimentin (prepared by our laboratory) was purified using protein A+G agarose (Beyotime).

Techniques: Infection

Journal: Parasites & Vectors

Article Title: Effects of host vimentin on Eimeria tenella sporozoite invasion

doi: 10.1186/s13071-021-05107-4

Figure Lengend Snippet: Vimentin protein expression levels in E. tenella sporozoite-infected DF-1 cells. a Western blotting of the internal reference GAPDH and vimentin protein. b Gray analysis of the relative expression level of the vimentin protein. ** P < 0.01 by Student’s t -test compared with the uninfected group

Article Snippet: Polyclonal rabbit antibody against vimentin (prepared by our laboratory) was purified using protein A+G agarose (Beyotime).

Techniques: Expressing, Infection, Western Blot

Journal: Parasites & Vectors

Article Title: Effects of host vimentin on Eimeria tenella sporozoite invasion

doi: 10.1186/s13071-021-05107-4

Figure Lengend Snippet: Total phosphorylation levels of vimentin in E. tenella sporozoite-infected DF-1 cells. a Western blotting of phosphorylated and unphosphorylated vimentin with a consistent level of total vimentin. b Gray analysis of the relative expression level of phosphorylated vimentin. * P < 0.05 and ** P < 0.01 Student’s t -test compared with the uninfected group

Article Snippet: Polyclonal rabbit antibody against vimentin (prepared by our laboratory) was purified using protein A+G agarose (Beyotime).

Techniques: Infection, Western Blot, Expressing

Journal: Parasites & Vectors

Article Title: Effects of host vimentin on Eimeria tenella sporozoite invasion

doi: 10.1186/s13071-021-05107-4

Figure Lengend Snippet: Changes in vimentin distribution after E. tenella sporozoite invasion in DF-1 cells

Article Snippet: Polyclonal rabbit antibody against vimentin (prepared by our laboratory) was purified using protein A+G agarose (Beyotime).

Techniques:

Journal: Parasites & Vectors

Article Title: Effects of host vimentin on Eimeria tenella sporozoite invasion

doi: 10.1186/s13071-021-05107-4

Figure Lengend Snippet: Effect of anti-vimentin polyclonal antibody on E. tenella sporozoite invasion in DF-1 cells. ** P < 0.01 by Student’s t -test compared with anti-vimentin polyclonal antibody treatment or with the same dose of normal rabbit IgG

Article Snippet: Polyclonal rabbit antibody against vimentin (prepared by our laboratory) was purified using protein A+G agarose (Beyotime).

Techniques:

Journal: Parasites & Vectors

Article Title: Effects of host vimentin on Eimeria tenella sporozoite invasion

doi: 10.1186/s13071-021-05107-4

Figure Lengend Snippet: Effects of vimentin downregulation on sporozoite invasion in DF-1 cells. a The expression of vimentin protein in different transfection groups. b Cell growth curves of different transfection groups. c The invasion rate of sporozoites after downregulation of vimentin. ** P < 0.01 by Student’s t -test compared with downregulated vimentin expression level or the untreated group

Article Snippet: Polyclonal rabbit antibody against vimentin (prepared by our laboratory) was purified using protein A+G agarose (Beyotime).

Techniques: Expressing, Transfection